First Trust NYSE® Arca® Biotechnology Index Fund (FBT)
Holdings of the Fund as of 5/13/2022

Total Number of Holdings (excluding cash): 30

Security Name Identifier CUSIP Classification Shares / Quantity Market Value Weighting Descending sort
Grifols, S.A. (ADR) GRFS 398438408 Health Care 4,593,295 $59,529,103.20 4.65%
Gilead Sciences, Inc. GILD 375558103 Health Care 796,420 $49,672,715.40 3.88%
QIAGEN N.V. QGEN N72482123 Health Care 1,054,793 $48,657,601.09 3.80%
Amgen Inc. AMGN 031162100 Health Care 193,566 $47,113,964.40 3.68%
United Therapeutics Corporation UTHR 91307C102 Health Care 256,920 $47,129,404.80 3.68%
Mettler-Toledo International Inc. MTD 592688105 Health Care 37,157 $46,636,122.27 3.65%
Biogen Inc. BIIB 09062X103 Health Care 231,232 $46,040,603.52 3.60%
BioMarin Pharmaceutical Inc. BMRN 09061G101 Health Care 591,884 $46,007,143.32 3.60%
Alkermes Plc ALKS G01767105 Health Care 1,689,542 $45,414,888.96 3.55%
Incyte Corporation INCY 45337C102 Health Care 605,246 $45,332,925.40 3.54%
Seagen Inc. SGEN 81181C104 Health Care 331,069 $44,902,888.47 3.51%
BioNTech SE (ADR) BNTX 09075V102 Health Care 281,072 $44,645,476.48 3.49%
Ionis Pharmaceuticals, Inc. IONS 462222100 Health Care 1,204,523 $43,459,189.84 3.40%
Regeneron Pharmaceuticals, Inc. REGN 75886F107 Health Care 67,249 $43,410,574.48 3.39%
Bio-Techne Corporation TECH 09073M104 Health Care 117,586 $43,244,603.22 3.38%
Exelixis, Inc. EXEL 30161Q104 Health Care 2,148,057 $43,132,984.56 3.37%
IQVIA Holdings Inc. IQV 46266C105 Health Care 206,098 $43,014,713.58 3.36%
Vertex Pharmaceuticals Incorporated VRTX 92532F100 Health Care 171,218 $42,242,904.96 3.30%
Neurocrine Biosciences, Inc. NBIX 64125C109 Health Care 497,235 $41,827,408.20 3.27%
FibroGen, Inc. FGEN 31572Q808 Health Care 4,119,049 $40,943,347.06 3.20%
Moderna, Inc. MRNA 60770K107 Health Care 296,633 $40,908,657.03 3.20%
Charles River Laboratories International, Inc. CRL 159864107 Health Care 172,485 $40,673,687.85 3.18%
Sarepta Therapeutics, Inc. SRPT 803607100 Health Care 605,284 $39,930,585.48 3.12%
Agios Pharmaceuticals, Inc. AGIO 00847X104 Health Care 1,767,786 $39,103,426.32 3.06%
Alnylam Pharmaceuticals, Inc. ALNY 02043Q107 Health Care 293,902 $37,169,785.94 2.91%
Exact Sciences Corporation EXAS 30063P105 Health Care 701,897 $36,702,194.13 2.87%
ACADIA Pharmaceuticals Inc. ACAD 004225108 Health Care 2,077,252 $35,001,696.20 2.74%
Illumina, Inc. ILMN 452327109 Health Care 146,735 $34,271,426.60 2.68%
Nektar Therapeutics NKTR 640268108 Health Care 7,966,147 $31,784,926.53 2.48%
Ultragenyx Pharmaceutical Inc. RARE 90400D108 Health Care 579,915 $29,848,225.05 2.33%
US Dollar $USD Other 1,699,600 $1,699,600.49 0.13%
Holdings are subject to change.

The holdings information set forth above for the date indicated reflects creation and redemption transactions from the prior business day and may differ from the holdings information currently available from the Fund's custodian and accounting agent. With respect to the market value of the Fund's holdings set forth above, the pricing sources are generally third party vendors. The Fund ultimately relies on pricing information provided by the Fund's accounting agent.